A rapid and quantitative flow cytometry method for the analysis of membrane disruptive antimicrobial activity

NM O'Brien-Simpson; N Pantarat; TJ Attard; KA Walsh; EC Reynolds

Journal article

A rapid and quantitative flow cytometry method for the analysis of membrane disruptive antimicrobial activity

NM O'Brien-Simpson, N Pantarat, TJ Attard, KA Walsh, EC Reynolds

Plos One | PUBLIC LIBRARY SCIENCE | Published : 2016

DOI: 10.1371/journal.pone.0151694

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Abstract

We describe a microbial flow cytometry method that quantifies within 3 hours antimicrobial peptide (AMP) activity, termed Minimum Membrane Disruptive Concentration (MDC). Increasing peptide concentration positively correlates with the extent of bacterial membrane disruption and the calculated MDC is equivalent to its MBC. The activity of AMPs representing three different membranolytic modes of action could be determined for a range of Gram positive and negative bacteria, including the ESKAPE pathogens, E. coli and MRSA. By using the MDC50 concentration of the parent AMP, the method provides high-throughput, quantitative screening of AMP analogues. A unique feature of the MDC assay is that it..

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University of Melbourne Researchers

Neil O'Brien-Simpson Author

Troy Attard Author

Katrina Walsh Author

Eric Reynolds Author

Related Projects (1)

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by the Australian Government, Department of Industry, Innovation and Science and the Australian National Health and Medical Research Council Project (Grant 1008106).